It is very important to reveal the evolution characteristics of ecosystem multi-function in the ancient Yellow River basin under the influence of comprehensive land consolidation to improve the sustainable ecosystem function and the optimal management of territorial space. To this end, this study selects the Suining section of the ancient Yellow River as the research area, and uses the InVEST model to comprehensively evaluate 8 ecosystem functions including primary production, human settlement carrying, recreational services, carbon sequestration, water production, water purification, soil conservation, and habitat maintenance. And reveal the change and trade-off-synergy relationship of ecosystem multifunctionality in the study area from 1990 to 2020. The research shows that: ①In the past 30 years, the land use in the study area has changed from agricultural production to a mode of use that takes into account production, living and ecology; ②In each ecosystem function, production and living functions such as primary production, human settlements, and recreational services are all common. While the functions of carbon sequestration, water production, water purification, soil conservation, and habitat maintenance have fluctuated and increased; ③The multi-functionality of ecosystem in the region increased by 21.07% in the past 30 years. ④ From a spatial point of view, there are significant synergistic relationships among the functions of habitat carrying-water production, habitat carrying-soil maintenance, carbon sequestration-habitat maintenance, while habitat carrying-carbon sequestration, habitat carrying-habitat maintenance, carbon sequestration. There are significant trade-offs among functions such as sequestration-water production, water production-habitat maintenance, and water purification-soil maintenance. Primary production-recreational services, primary production-water production, and primary production-habitat maintenance gradually evolved into a synergistic relationship. This study shows that the comprehensive improvement of the ancient Yellow River has a significant positive effect on the multi-functionality of the ecosystem, and synergistically improves the production-life-ecological functions (intensification of agricultural production, intensification of residents’ carrying capacity, ecologicalization of the watershed, intensification of production, concentration of life, and ecological restoration).is an effective way for the sustainable development of the ancient Yellow River ecosystem. In the future, regional ecosystem adaptive management should be strengthened to improve ecosystem sustainability.