The groundwater buried in the mining area of eastern China is shallow, and the reclamation of coal mining subsidence is mostly by agricultural and fishing reclamation. However, the widespread use of antibiotics in the aquaculture industry has caused contamination of antibiotic resistance genes (antibiotic resistance genes (ARGs)).To explore the pollution characteristics of ARGs in agricultural and fishery reclamation subsidence areas, the abundance and composition of ARGs were analyzed using metagenomics technology.There were 957 ARGs in the reclaimed agricultural soil samples, with the most widespread distribution of MacB and tetA (58). It found that the composition and relative abundance of ARGs in reclaimed agricultural soils were higher than in ordinary agricultural soils, and increased with soil depth. Reclamation agricultural soils show greater ARGs capacity and ARGs are able to migrate to deeper soil and accumulate. 21 subtypes of ARGs were found, with the highest content of multidrug ARGs, MLS ARGs and tetracycline ARGs. There were 29 ARGs in the fish pond sediments, with widespread distribution of MacB. The species and relative abundance of ARGs in the sediments were much lower than in the soil. In addition, the relative abundance of sulfamamine ARGs and tetracycline ARGs was high and multidrug ARGs were abundant in well water samples. In total, 20 subtypes of ARGs were found, of which the relative abundance of multidrug ARGs was the highest, at approximately 31.1%.There were 15 classes of ARGs subtypes in soil and sediment, with a similar composition. The relative abundance of most ARGs isoforms in the soil samples was significantly higher than the fish pond sediment samples, such as the glycopeptide ARGs and the tetracycline ARGs.

antibiotic resistance genes,metagenomics,agricultural and fishing reclamation,sediment,soil